ea0028oc5.6 | Growth, tumours and pituitary | SFEBES2012
Mirczuk Samantha
, Wilkinson Samantha
, Savage Alyssa
, Sunters Andrew
, Simbi Bigboy
, McArdle Craig
, Lee Martin
, Fowkes Robert
The orphan nuclear receptor, SF-1, is sumoylated in adrenal and gonadal tissues, but the role of sumoylation in pituitary gonadotrophs is currently unknown. We performed expression studies for key components of sumoylation machinery in αT3-1 and LβT2 gonadotroph cell lines, using RT-PCR. Expression of the genes encoding the SUMO proteins (Sumo1, Sumo2, Sumo3) as well as the E3 ligase enzymes responsible for their covalent linkage (Pias1, Pias2/xα, Pias...